## eDNA Sampling Details

Report Number: 2504241105

Receiving Entity: New York State Department of Environmental Conservation

Location: Oak Orchard Creek, NY

Collection Entity (Office): U.S. Fish and Wildlife Service (Lower Great Lakes FWCO)

Sample Date(s): Jul. 24, 2024

eDNA Samples Processed: 12 environmental samples, 1 controls

Target Gene: 12s (Genomic site used for DNA amplification)

### Quick Glance Results

- A total of 12 unique sample locations were surveyed at Oak Orchard Creek, NY in the Lake Ontario basin.

- Across all samples, DNA was detected from 43 taxa including a total of 40 unique species-level detections.

- On average per sample, the amount of water filtered was 1.86 liters, the sequencing depth (number of assigned DNA reads) was 182,330 reads, and the number of detected taxa was 28 species and genera.

## Great Lakes USFWS AIS Watchlist Detections

The USFWS Great Lakes AIS watchlist was developed utilizing information from Great Lakes Aquatic Nonindigenous Species Information System (GLANSIS), USFWS Ecological Risk Assessments, the Great Lakes and St. Lawrence Governors and Premiers Least Wanted list, and partner input.

AIS Watchlist Species Were Detected
Common Name | Scientific Name | Determination | Number of Samples with DNA Detected
--- | --- | --- | ---
Grass Carp | Ctenopharyngodon idella | Detection | 1
Rudd | Scardinius erythrophthalmus | Detection | 9
Note: Use dropdown list in the map below to see where exactly at this location watchlist detections occurred.

## eDNA Results

#### Location Map

The results map is interactive and can be used to explore sample locations and where specific taxa DNA detections occurred within Oak Orchard Creek, NY. Layers can be turned on/off by checking the boxes in the top right corner of the map. To see where DNA detections occurred for a specific taxa, select a species from the drop-down list and ensure the Metabarcoding Results layer is added to the map. When all species are selected only information for the topmost record is displayed. Clicking on an individual point will open a pop-up that contains information regarding that particular record. Additionally, the map can be enlarged by clicking the expand icon in the top left corner of the map, and saved as an image file by selecting the download icon also in the top left corner.

Fish Taxa Name:

Figure 1. Interactive map showing sample locations (yellow circles), metabarcoding detections (blue squares), qPCR results (If present: purple triangle = negative detection, orange inverted-triangle = positive detction), and Non-indiginous Aquatic Species (NAS) records for associated HUC10 drainages (If present: green diamond). Metabarcoding, qPCR, and NAS layers are able to be filtered by a selected taxa. Clicking on an individual point in a layer will open a pop-up that contains information for that sample.

#### Detection Table

Table 1. The eDNA detection summary table lists all taxa DNA detected. The table includes the taxon name (common and scientific), along with the number of samples in which it was detected, the percentage of all samples in which it was detected, and the mean sequence read frequency of those detections. The table is able to be sorted in either ascending or descending order by clicking on a column header.

## Accessing eDNA Results Data

### Report Data

The data associated with this report can be downloaded for further exploration or analysis. The file will download as an Excel file with a second worksheet that contains a data dictionary providing descriptions of the data types and fields found in the dataset.

### Complete Great Lakes Metabarcoding Dataset

The Great Lakes Early Detection and Monitoring Programs metabarcoding results dataset can be found here. This file is updated when results are publicly posted following the communications protocol.

## Questions and Additional Information

For any questions related to detection results or assistance with interpretation please contact the following program lead:

Nick Frohnauer
  USFWS, Great Lakes EDM Coordinator | email: nicholas_frohnauer@fws.gov | phone: 612-431-5829