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Pacific Lamprey eDNA Detection Summary Data 7-17-24

Report Generated: 2025-02-18 21:39:38.289610445 +0000

Metadata Source

Metadata schema: mdJson
Schema version: 2.8.1
Contacts
Patrick DeHaan
Contact ID: CID001
is Organization: false
Contact Type: federal
Position Name: Project Leader
is Member of: Whitney Genetics Lab
Phone
Phone Number: 608-518-6133
Service Types: mobile
Electronic Mail: patrick_dehaan@fws.gov
Online Resource
URI: https://www.fws.gov/office/whitney-genetics-laboratory
Name: Whitney Genetics Lab Website
Whitney Genetics Lab
Contact ID: CID002
is Organization: true
Contact Type: federal
Address
555 Lester Ave
Onalaska, WI 54650
USA
Address Type: mailing
Address Type: physical
Online Resource
URI: https://www.fws.gov/office/whitney-genetics-laboratory
Name: Lab Website
Western Washington Fish and Wildlife Conservation Office
Contact ID: 88304f63-47b1-47b9-be05-f4add05e9965
is Organization: true
Contact Type: federal
Address
500 Desmond Dr. SE
Lacey, WA 98503
USA
Address Type: mailing
Address Type: physical
Online Resource
URI: https://www.fws.gov/office/western-washington-fish-and-wildlife-conservation
Name: Station Website
Metadata Information
Metadata Status: final
Metadata Identifier
Identifier: 6499a1dd-c1b4-4f55-beea-3555c74ce0a4
Namespace: urn:uuid
Metadata Locales
Default Locale
Language Code: eng
Country Code: USA
Character Encoding: UTF-8
Metadata Contacts
author
Patrick DeHaan
Contact ID: CID001
Contact Type: federal
Position Name: Project Leader
Metadata Dates
Date: creation: 2025-02-18T06:00:00.853+00:00
Resource Information
Resource Type: tabularDataset Name: Pacific Lamprey Summary Data 7-17-24.csv
Status: final
Status: completed
Citation
Title: Pacific Lamprey eDNA Detection Summary Data 7-17-24
Abstract
Full Abstract:
qPCR detection results for Pacific Lamprey eDNA surveys from Western Washington Rivers and Streams. Water samples were collected via filtration and shipped to the laboratory for analysis. DNA was extracted from filters using commercially available extraction kits. Lab staff used two existing Pacific Lamprey qPCR eDNA surveys to screen DNA extracts for the presence of Pacific Lamprey DNA. Eight molecular replicates were run for each samples. Results presented in this table show the number of positive detections out of 8 for each sample and the corresponding mean Cq values for each sample with a detection.

Purpose, Usage, and Limitations
Purpose:
This is the final eDNA detection dataset for the project.

Resource Contacts
author
Patrick DeHaan
Contact ID: CID001
Contact Type: federal
Position Name: Project Leader
author
Whitney Genetics Lab
Contact ID: CID002
Contact Type: federal
author
Western Washington Fish and Wildlife Conservation Office
Contact ID: 88304f63-47b1-47b9-be05-f4add05e9965
Contact Type: federal
Temporal Information
Time Period
Start Datetime: 2021-07-01T05:00:00.000+00:00
End Datetime: 2025-01-31T21:24:23.375+00:00
Description:
Project Period of Performance
Time Interval
Interval: 5
Units: year
Spatial, Temporal, and Vertical Extents
Extent
Geographic Extent
Geographic Extent Encompasses Data: true
Geographic Description:
Rough approximation of our study area, Puget Sound and Coastal Washington
Map
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Keywords
theme
  • Science Keywords > EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH
  • Science Keywords > EARTH SCIENCE > OCEANS > AQUATIC SCIENCES > FISHERIES
  • Science Keywords > EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > CONSERVATION
  • Science Keywords > EARTH SCIENCE > AGRICULTURE > ANIMAL SCIENCE > ANIMAL BREEDING AND GENETICS
Thesaurus
Title: Global Change Master Directory (GCMD) Science Keywords
Date: revision: 2018-12-12
Edition: Version 8.6
Identifier
Identifier: 1eb0ea0a-312c-4d74-8d42-6f1ad758f999
Online Resource
URI: https://earthdata.nasa.gov/gcmd-forum
Resource Locales
Default Locale
Language Code: eng
Country Code: USA
Character Encoding: UTF-8
Data Quality
Data Dictionaries
Dictionary
Dictionary Description:
This is the data dictionary used for the Pacific Lamprey eDNA detection dataset.
Subject: tabularDataset
Entities
Pacific Lamprey eDNA Detection Data Dictionary
ID: 6de8ecb0-2cc8-4e23-8e10-a4c9bd66b361
Code: Pacific Lamprey eDNA Detection Data Dictionary
Definition:
The data dictionary for our Pacific Lamprey eDNA dataset
Attribute: Sample
Code: Sample
Definition:
The unique ID for each water filter sample
Datatype: integer
Allow NULL Values: false
Attribute Values Must Be Unique: true
Value is Case Sensitive: false
Attribute: Collection Date
Code: Collection Date
Definition:
Date the sample was collected
Datatype: character varying
Allow NULL Values: false
Attribute Values Must Be Unique: true
Value is Case Sensitive: false
Attribute: Location
Code: Location
Definition:
The name of the watershed the sample was collected from
Datatype: character varying
Allow NULL Values: false
Attribute Values Must Be Unique: true
Value is Case Sensitive: false
Attribute: Number of Filters Used
Code: Number of Filters Used
Definition:
The number of filters required to process 5L of water for this sample
Datatype: integer
Allow NULL Values: false
Attribute Values Must Be Unique: true
Value is Case Sensitive: false
Attribute: EntTri Number Positive Replicates
Code: EntTri Number Positive Replicates
Definition:
The number of positive molecular replicates (out of 8) for the EntTri marker
Datatype: integer
Allow NULL Values: false
Attribute Values Must Be Unique: true
Value is Case Sensitive: false
Attribute: EntTri Mean Cq Value
Code: EntTri Mean Cq Value
Definition:
The mean Cq value (averaged over 8 molecular replicates) for the EntTri marker
Datatype: integer
Allow NULL Values: false
Attribute Values Must Be Unique: true
Value is Case Sensitive: false
Attribute: PLamp Number Positive Replicates
Code: PLamp Number Positive Replicates
Definition:
The number of positive molecular replicates (out of 8) for the PLamp marker
Datatype: integer
Allow NULL Values: false
Attribute Values Must Be Unique: true
Value is Case Sensitive: false
Attribute: PLamp Mean Cq Value
Code: PLamp Mean Cq Value
Definition:
The mean Cq value (averaged over 8 molecular replicates) for the PLamp marker
Datatype: integer
Allow NULL Values: false
Attribute Values Must Be Unique: true
Value is Case Sensitive: false
Attribute: Notes
Code: Notes
Definition:
Any notes or comments about this particular sample
Datatype: unknown
Allow NULL Values: true
Attribute Values Must Be Unique: true
Value is Case Sensitive: false
Citation
Title: Pacific Lamprey eDNA Detections Data Dictionary
Date: creation: 2025-02-18T21:30:15.899+00:00
author
Patrick DeHaan
Contact ID: CID001
Contact Type: federal
Position Name: Project Leader
Whitney Genetics Lab
Contact ID: CID002
Contact Type: federal
Western Washington Fish and Wildlife Conservation Office
Contact ID: 88304f63-47b1-47b9-be05-f4add05e9965
Contact Type: federal
Dictionary Included with Dataset?: false